Figure 6.

CD4⁺ T cells are not required for the recovery of CD8⁺ T cells within corticosterone-treated ganglia. Mice latently infected with HSV-1 received 4 days of restraint stress and corticosterone treatment (30-34 dpi). At 27 and 30 dpi mice received intraperitoneal injections of 0.75 mg/ml ¿-CD4 monoclonal antibody (clone GK1.5) or a control antibody. TG were excised at 38 dpi and stained for CD8¿, CD4, CD45, and gB-specific T cell receptor. (A) Represenative dot plots demonstrate effective depletion of CD4⁺ T cells in the TG at 34 and 38 dpi. (B) Quantification of the CD4 depletion in mock depleted and CD4-depleted groups. (C) TG were excised at 38 dpi and analysed for recovery of the CD8⁺ T cell population. Data are presented as means and standard errors of 15 (mock depleted) or 16 (CD4-depleted) mice from 3 independent experiments. Students t test revealed no significant differences in the number of CD8⁺ T cells within TG from the mock depleted or CD4-depleted groups.